Description

Intended use

The ExtraStar^® Purification Kit 2.0 uses magnetic particle technology and is intended to be used for the automated isolation and purification of nucleic acids from human respiratory swab specimens for in vitro diagnostic purposes. The product is designed for use with the KingFisher™ Flex Purification System (Thermo Fisher Scientific) and altona Diagnostics kits and reagents specified for use with the ExtraStar^® Purification Kit 2.0.

Kit content

All reagents included in the ExtraStar^® Purification Kit 2.0 are ready-to-use solutions and are shipped in two separate boxes: Box 1 and Box 2. The boxes are shipped at room temperature.

 

Box 1 has to be stored at +15 °C to +30 °C and

Box 2 has to be stored at +2 °C to +8 °C upon receipt.

 

The ExtraStar^® Purification Kit 2.0 contains reagents sufficient for 384 sample purifications with a 300 µl sample volume.

ExtraStar® Purification Kit 2.0 – Content of Box 1

 

Box 1 ExtraStar^® Purification Kit 2.0: This box contains 8 bottles, 2 bottle of each component. The Lysis Buffer contains chaotropic salts (guanidine thiocyanate,octoxynol 9) to disrupt cells or virions chemically. It stabilizes nucleic acids and protects them against nucleases in solution. The Wash Buffer 1 contains different salts and organic solvents (guanidine thiocyanate, ethanol) to remove proteins and other impurities. The Wash Buffer 2 contains organic solvents (ethanol) to remove proteins and other impurities. The Wash Buffer 3 contains different salts in order to purify the nucleic acids.

ExtraStar® Purification Kit 2.0 – Content of Box 2

Box 2 ExtraStar^® Purification Kit 2.0: The Enhancer stabilizes and protects nucleic acids against nuclease in solution. The Magnetic Beads are coated with a thin layer of silica to bind nucleic acids in solution. The magnetic characteristic allows the separation of beads from liquids in magnetic field. The Elution Buffer is a low salt buffer to elute the nucleic acids from the magnetic beads for subsequent analysis.